analysis of Egg white proteins

METHOD Use third person, past tense. Do not copy the prac book word for word, just include the important information. No dot points. All important values must be present, including composition of buffers. Tables may be presented as long as they include relevant UNITS. Include the method for preparation of the egg white protein (section 1.1). DISCUSSION Isolation of ovalbumin Discuss your results. ‚· Could you observe a band for ovalbumin in your sample? ‚· Were there any other proteins co-purified in this experiment? ‚· Can you identify any of those other proteins? ‚· Can you roughly calculate the % purity of your ovalbumin sample? ‚· Comment on what type of proteins might be co-purified using this method and how could you possibly increase the purity of your sample further? ‚· Specifically describe how you would apply another method to improve this isolation. Determination of lysozyme activity of ion exchange fractions: Discuss your results. ‚· What did you expect in each of the lysozyme fractions? ‚· Did the results agree with your expectations? ‚· Can you explain what happened relating to the biochemical principles? ‚· How well did the purification of lysozyme with ion exchange work in terms of % recovery and % purity? ‚· Why do you think the Fraction B and Fraction C sample scored some activity when it theoretically should not contain any lysozyme? ‚· Did the samples progressively decrease in total activity, but increase in specific activity? ‚· Discuss any limitations of the experiment. For full marks, you will also need to cite relevant references in your discussion of your results. CONCLUSION Give the conclusion, including: ‚· Approximate % purity of ovalbumin sample ‚· Total lysozyme activity isolated ‚· Specific lysozyme activity and approximate % purity References Use reputable sources such as textbooks and journal articles. Wikipedia is NOT a reputable source of information. Note the formatting styles for referencing. References should be cited in the introduction and discussion sections if relevant. For a custom paper on the above topic, place your order now! What We Offer: ¢ On-time delivery guarantee ¢ PhD-level writers ¢ Automatic plagiarism check ¢ 100% money-back guarantee ¢ 100% Privacy and Confidentiality ¢ High Quality custom-written papers